Abstract
Several phorbol esters are potent activators of protein kinase C. They down-regulate gap junctional intercellular communication and induce phosphorylation of connexin43, but the sensitivity and extent of responses vary much between systems. We asked whether the total protein kinase C enzyme activity or the protein kinase C isoenzyme constitution was of importance for such variations. Some fibroblastic culture systems were compared. It was concluded that the total protein kinase C enzyme activity did not determine the sensitivity to phorbol esters. Furthermore, the use of isotype-specific inhibitors of protein kinase C indicated that protein kinase Cα, δ, and ε may be involved to different extents in different fibroblastic systems in the response to phorbol esters.
Original language | English |
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Pages (from-to) | 150-161 |
Number of pages | 11 |
Journal | Experimental Cell Research |
Volume | 268 |
DOIs | |
Publication status | Published - Aug 2001 |
Externally published | Yes |
Keywords
- protein kinase C
- gap junctional intercellular communication
- connexin43