Microinjection of HEp-2 cells with coxsackie B1 virus RNA enhances invasiveness of Shigella flexneri only after prestimulation with UV-inactivated virus

Kristin R. Modalsli; Svein Ole Mikalsen; Geir Bukholm; Miklos Degré

doi:10.1111/j.1699-0463.1993.tb00153.x

Microinjection of HEp-2 cells with coxsackie B1 virus RNA enhances invasiveness of Shigella flexneri only after prestimulation with UV-inactivated virus

Kristin R. Modalsli, Svein Ole Mikalsen, Geir Bukholm, Miklos Degré

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Coxsackie B1 virus induces increased susceptibility to invasion by Shigella flexneri when HEp-2 cells are inoculated with the complete virus. When RNA from the same virus was microinjected into cells, virus RNA was synthesized and new virus particles were formed, but the transfected RNA had no effect on bacterial invasiveness. However, when the cells were prestimulated with UV-inactivated virus, the microinjected RNA induced an additional enhancement of bacterial invasiveness. Microinjected whole virus particles did not replicate and did not induce any change in bacterial invasiveness. The results indicate that an initial event in virus multiplication is necessary to achieve an effect of transfected viral RNA on invasion of S. flexneri.

Original language	English
Pages (from-to)	602-606
Number of pages	5
Journal	APMIS. Journal of pathology, microbiology and immunology
Volume	101
DOIs	https://doi.org/10.1111/j.1699-0463.1993.tb00153.x
Publication status	Published - 1993
Externally published	Yes

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10.1111/j.1699-0463.1993.tb00153.x

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@article{5d950fd2fe214ddc997230c820ae0a2a,

title = "Microinjection of HEp-2 cells with coxsackie B1 virus RNA enhances invasiveness of Shigella flexneri only after prestimulation with UV-inactivated virus",

abstract = "Coxsackie B1 virus induces increased susceptibility to invasion by Shigella flexneri when HEp-2 cells are inoculated with the complete virus. When RNA from the same virus was microinjected into cells, virus RNA was synthesized and new virus particles were formed, but the transfected RNA had no effect on bacterial invasiveness. However, when the cells were prestimulated with UV-inactivated virus, the microinjected RNA induced an additional enhancement of bacterial invasiveness. Microinjected whole virus particles did not replicate and did not induce any change in bacterial invasiveness. The results indicate that an initial event in virus multiplication is necessary to achieve an effect of transfected viral RNA on invasion of S. flexneri.",

author = "Modalsli, {Kristin R.} and Mikalsen, {Svein Ole} and Geir Bukholm and Miklos Degr{\'e}",

year = "1993",

doi = "10.1111/j.1699-0463.1993.tb00153.x",

language = "English",

volume = "101",

pages = "602--606",

journal = "APMIS. Journal of pathology, microbiology and immunology",

issn = "0903-4641",

publisher = "John Wiley & Sons Inc.",

}

Microinjection of HEp-2 cells with coxsackie B1 virus RNA enhances invasiveness of Shigella flexneri only after prestimulation with UV-inactivated virus. / Modalsli, Kristin R.; Mikalsen, Svein Ole; Bukholm, Geir et al.
In: APMIS. Journal of pathology, microbiology and immunology, Vol. 101, 1993, p. 602-606.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Microinjection of HEp-2 cells with coxsackie B1 virus RNA enhances invasiveness of Shigella flexneri only after prestimulation with UV-inactivated virus

AU - Modalsli, Kristin R.

AU - Mikalsen, Svein Ole

AU - Bukholm, Geir

AU - Degré, Miklos

PY - 1993

Y1 - 1993

N2 - Coxsackie B1 virus induces increased susceptibility to invasion by Shigella flexneri when HEp-2 cells are inoculated with the complete virus. When RNA from the same virus was microinjected into cells, virus RNA was synthesized and new virus particles were formed, but the transfected RNA had no effect on bacterial invasiveness. However, when the cells were prestimulated with UV-inactivated virus, the microinjected RNA induced an additional enhancement of bacterial invasiveness. Microinjected whole virus particles did not replicate and did not induce any change in bacterial invasiveness. The results indicate that an initial event in virus multiplication is necessary to achieve an effect of transfected viral RNA on invasion of S. flexneri.

AB - Coxsackie B1 virus induces increased susceptibility to invasion by Shigella flexneri when HEp-2 cells are inoculated with the complete virus. When RNA from the same virus was microinjected into cells, virus RNA was synthesized and new virus particles were formed, but the transfected RNA had no effect on bacterial invasiveness. However, when the cells were prestimulated with UV-inactivated virus, the microinjected RNA induced an additional enhancement of bacterial invasiveness. Microinjected whole virus particles did not replicate and did not induce any change in bacterial invasiveness. The results indicate that an initial event in virus multiplication is necessary to achieve an effect of transfected viral RNA on invasion of S. flexneri.

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U2 - 10.1111/j.1699-0463.1993.tb00153.x

DO - 10.1111/j.1699-0463.1993.tb00153.x

M3 - Article

SN - 0903-4641

VL - 101

SP - 602

EP - 606

JO - APMIS. Journal of pathology, microbiology and immunology

JF - APMIS. Journal of pathology, microbiology and immunology

ER -

Microinjection of HEp-2 cells with coxsackie B1 virus RNA enhances invasiveness of Shigella flexneri only after prestimulation with UV-inactivated virus

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