MassSorter: Peptide Mass Fingerprinting Data Analysis: Functional Proteomics - Methods and Protocols

Ingvar Eidhammer; Harald Barsnes; Svein-Ole Mikalsen

doi:10.1007/978-1-59745-398-1_23

MassSorter: Peptide Mass Fingerprinting Data Analysis: Functional Proteomics - Methods and Protocols

Ingvar Eidhammer, Harald Barsnes, Svein-Ole Mikalsen

Náttúruvísindadeildin - Faculty of Science and Technology

Research output: Other contribution

1 Citation (Scopus)

Abstract

MassSorter is a software tool that sorts, systemizes, and analyzes data from peptide mass fingerprinting (PMF) experiments on proteins with known amino acid sequences. Several experiments can be simultaneously analyzed for sequence coverage and posttranslational modifications occurring during sample handling, induced chemical modifications, and unexpected cleavages. Experimental m/z values are compared with m/z values from an in silico digestion, taking modifications into account. Filters can be defined by users for marking autolytic protease peaks and other contaminating peaks. MassSorter functions as a database of all the detected peptides. It includes tools for visualization of the results, such as sequence coverage, accuracy plots, statisties, and 3D models.

Original language	English
Publisher	Humana Press / Springer Science Media
Number of pages	15
Volume	484
ISBN (Print)	978-1-58829-971-0
ISBN (Electronic)	978-1-59745-398-1
DOIs	https://doi.org/10.1007/978-1-59745-398-1_23
Publication status	Published - 2008

Publication series

Name	Methods in Molecular Biology
Publisher	Humana Press / Springer Science Media
Volume	484

Keywords

Peptide mass fingerprinting
MassSorter
analyzing MS data
comparing MS experiments

Access to Document

10.1007/978-1-59745-398-1_23

Cite this

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title = "MassSorter: Peptide Mass Fingerprinting Data Analysis: Functional Proteomics - Methods and Protocols",

abstract = "MassSorter is a software tool that sorts, systemizes, and analyzes data from peptide mass fingerprinting (PMF) experiments on proteins with known amino acid sequences. Several experiments can be simultaneously analyzed for sequence coverage and posttranslational modifications occurring during sample handling, induced chemical modifications, and unexpected cleavages. Experimental m/z values are compared with m/z values from an in silico digestion, taking modifications into account. Filters can be defined by users for marking autolytic protease peaks and other contaminating peaks. MassSorter functions as a database of all the detected peptides. It includes tools for visualization of the results, such as sequence coverage, accuracy plots, statisties, and 3D models.",

keywords = "Peptide mass fingerprinting, MassSorter, analyzing MS data, comparing MS experiments",

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AU - Mikalsen, Svein-Ole

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N2 - MassSorter is a software tool that sorts, systemizes, and analyzes data from peptide mass fingerprinting (PMF) experiments on proteins with known amino acid sequences. Several experiments can be simultaneously analyzed for sequence coverage and posttranslational modifications occurring during sample handling, induced chemical modifications, and unexpected cleavages. Experimental m/z values are compared with m/z values from an in silico digestion, taking modifications into account. Filters can be defined by users for marking autolytic protease peaks and other contaminating peaks. MassSorter functions as a database of all the detected peptides. It includes tools for visualization of the results, such as sequence coverage, accuracy plots, statisties, and 3D models.

AB - MassSorter is a software tool that sorts, systemizes, and analyzes data from peptide mass fingerprinting (PMF) experiments on proteins with known amino acid sequences. Several experiments can be simultaneously analyzed for sequence coverage and posttranslational modifications occurring during sample handling, induced chemical modifications, and unexpected cleavages. Experimental m/z values are compared with m/z values from an in silico digestion, taking modifications into account. Filters can be defined by users for marking autolytic protease peaks and other contaminating peaks. MassSorter functions as a database of all the detected peptides. It includes tools for visualization of the results, such as sequence coverage, accuracy plots, statisties, and 3D models.

KW - Peptide mass fingerprinting

KW - MassSorter

KW - analyzing MS data

KW - comparing MS experiments

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DO - 10.1007/978-1-59745-398-1_23

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VL - 484

T3 - Methods in Molecular Biology

PB - Humana Press / Springer Science Media

ER -