Circuit Topology Analysis of Single-Cell HiC Data

Barbara Scalvini, Alireza Mashaghi

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

The 3D fold structure of the genome is intricately linked to its function. As a result, descriptors of 3D genome conformation are becoming increasingly important as markers for disease and therapeutic responses. Circuit topology, a theory of folds, formalizes the arrangement of contacts in an entangled chain. It is uniquely suited for the topological description of the cellular genome and changes to genomic architecture during physiological processes like cellular differentiation or pathological and therapeutic alterations. In this discussion, we will explore circuit topology and its ability to extract topological information from single-cell HiC data.
Original languageEnglish
Title of host publicationBacterial Chromatin
Subtitle of host publicationMethods and Protocols
EditorsRemus T. Dame
Place of PublicationNew York
PublisherHumana New York, NY
Pages27-38
Number of pages12
ISBN (Electronic)978-1-0716-3930-6
ISBN (Print)978-1-0716-3929-0
Publication statusPublished - 20 Jul 2024

Publication series

NameMethods in Molecular Biology
PublisherHumana New York, NY
Number2819
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Microbiology
  • Methods
  • Topology
  • Circuit topology
  • 3D genome
  • HiC
  • Single-cell analysis

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